Jump to content Jump to navigation menu

Technical Data Sheet
1NNP 1NN7 1NNP_offen

anti-HA-tag mouse monoclonal, 12CA5, 25 µg
From  PROGEN
lyophilized, monoclonal, affinity purified
Icon_Abweichende-Konditionen
Related products
Pack Qty.
Pack.
Primary antibodies
Storage temp. +4 °C
Transport temp. ambient temp.

Binding of HA-tag proteins
0.25 mg/ml after reconstitution with 100 μl / 400 μl PBS

Specific primary antibodies that recognise the most commonly used protein tags. The antibodies can be excellently used in cell or tissue biology studies, protein-protein interaction assays, post-translational modifications, expression dynamics or localisation studies, up to the production of analytical kits in laboratory or large scale.
Product details

€117.20/Pack Qty. 

excl. VAT. | 25 µg per Pack Qty.

Art. No. 1NN7.1

Available
Ready for dispatch in 1-2 weeks
from 6 Pack Qty. €111.34/Pack Qty.
from 24 Pack Qty. €105.48/Pack Qty.

Fits to / Accessories

1YE6
Chemiluminescence documentation system chemiLITE
Art. No. 1YE6.1
€15,517.65  /  1  unit(s) 
HP40
Transfer membrane ROTI®NC 0.2, 300 × 30 cm
Art. No. 1YXN.1
€405.30  /  1  roll(s) 
Transfer membrane ROTI<sup>&reg;</sup>PVDF 0.2, 300 × 30 cm
Transfer membrane ROTI®PVDF 0.2, 300 × 30 cm
Art. No. 1YYC.1
€475.15  /  1  roll(s) 
A151
ROTI®Block, 250 ml
10x conc., ready-to-use
Art. No. A151.1
€64.40  /  250  ml 
P078
ROTI®Lumin, 2 x 120 ml
10x conc., fluorescence-free, for protein and cytoimmunochemistry
Art. No. P078.1
€504.20  /  1  kit 
9211_9213_9214
Signal Enhancer Western/ELISA Set, 2 x 250 ml
for immunochemistry
Art. No. 9211.1
€489.15  /  1  kit 
1059
ROTI®Stock 10x PBST
10x conc., BioScience Grade, ready-to-use, sterile filtered
Art. No. 1059.1
€155.90  /  1  l 
8862
Western Incubation Trays, MIDI
Art. No. 8870.1
€289.20  /  10  unit(s) 
0509_0070
Antibody Diluent green, 125 ml, 1 x 125 ml
ready-to-use, for immunohistochemistry
Art. No. 0070.1
€89.15  /  125  ml 
2245_Bande_ai
ROTI®-Mark WESTERN PLUS, 250 µl, 1 x 250 µl
ready-to-use, pre-dyed
Art. No. 2245.1
€252.65  /  250  µl 

Product details


Specific primary antibodies that recognise the most commonly used protein tags. The antibodies can be excellently used in cell or tissue biology studies, protein-protein interaction assays, post-translational modifications, expression dynamics or localisation studies, up to the production of analytical kits in laboratory or large scale.

  • Available for most commonly used protein tags (His, DDDDK, GST, HA, myc, GFP & Strep)
  • High affinity to the respective protein tag
  • Robust & highly specific detection of the fusion protein
  • Unconjugated
  • Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence


anti-HA-tag mouse monoclonal, 12CA5 lyophilized, monoclonal, affinity purified

The monoclonal 12CA5 antibody recognizes the HA-tag (hemagglutinin tag). The HA-tag is commonly added to recombinant proteins and can be used for detection or purification of the tagged protein.

Host: mouse
Antibody type: monoclonal
Isotype: IgG2b
Clone: 12CA5
Immunogen: 9 amino acids from the human influenza virus hemagglutinin protein
Conjugate: unconjugated
Purification: affinity chromatography
Intended use: research use only
Application: WB
Reactivity: HA


Application

Western Blot (WB): 1:50,000-1:100,000 (0.005-0.0025 μg/ml)


1NN7
Western blot analysis of E. coli lysate containing HA-tagged protein with anti-HA-tag antibody. Western blot analysis was performed on 10 ng or 5 ng of E. coli lysate containing HA-tagged protein. Cells were lysed with SDS sample buffer. The PVDF membrane was blocked with 5 % dry milk in PBST for 1 h at RT. The primary antibody anti-HA-tag mouse monoclonal, 12CA5 (Cat. No. 910HAL) was diluted in blocking buffer (antibody concentration 0.005 μg/ml) and incubated for 1 h at RT. The secondary antibody goat anti-mouse IgG polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.


Not a medical device / Not an IVD product
anti-HA-tag mouse monoclonal, 12CA5
Selected quantity:   0
  1. Subtotal:  0.00
Art. No. Pack Qty. Pack. Price Quantity
1NN7.1 25 µg plastic

€117.20

1NN7.2 100 µg plastic

€385.95

In stock
Available
In procurement
No longer available
Delivery date currently unknown
Selected quantity:   0
  1. Subtotal:  0.00

Downloads / MSDS

Instruction / German
Specification / German
Instruction / English
Specification / English
Specification / French
Security datasheet
Germany / German
Security datasheet
International / English

Certificates of Analysis

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found: