Technical Data Sheet
Transfer membrane ROTI®NC 0.45, 300 × 30 cm
High-quality transfer membrane for all detection systems - extremely suitable for all protein transfer and detection methods. The membrane stands out for its particularly high mechanical stability. The pure 100 % nitrocellulose quality membrane is easy to block and allows for background-free blots with perfect bands.
€406.35/Pack Qty.
excl. VAT. | 1 roll(s) per Pack Qty.
Art. No. 200K.1
Fits to / Accessories
Product details
Transfer membrane for protein analyses, Southern- and Northern-Blots.
High-quality transfer membrane for all detection systems - extremely suitable for all protein transfer and detection methods. The membrane stands out for its particularly high mechanical stability. The pure 100 % nitrocellulose quality membrane is easy to block and allows for background-free blots with perfect bands.
Transfer membrane ROTI®NC 0.45
The pore size of 0,45 μm is suitable for proteins and peptides of all sizes. The best retention rate is achieved when blotting proteins of ≥20 kDa. Having a high protein binding capacity, the membrane not only efficiently prevents protein loss during transfer, but also guarantees very sensitive detection of even low protein amounts.
A slight optical difference between the two membrane sides results from the membrane production and has no influence on the binding capacity or the blot quality of the two sides. Both sides can be used equally for transfer and detection.We recommend to always use the optically same side as “detection side” to achieve maximum reproducibility.
Optimised for proteins ≥20 kDa. Western- and far-Western-blots, Southern- and Northern blots, colony- and plaque-lifting of nucleic acids and proteins, dot- and slot blots.
Detection via radio activity, chemoluminescence, colour.
- Western blotting
- Protein and immunoblotting
- Northern blotting
- Southern blotting
- Dot / slot blotting
| Dimensions | 300 × 30 cm |
| Version | roll |
| Pore size | 0,45 µm |
| Bond | hydrophobic |
| Binding capacity | 100 µg/cm² |
| Thickn. | 150±50 µm |
| Immobilisation | UV, baking (max. 80 °C) |
- Subtotal: 0.00
| Art. No. | Dimensions | Pack Qty. | Price | Quantity | |
|---|---|---|---|---|---|
| 200K.1 | 300 × 30 cm | 1 roll(s) |
€406.35 |
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| 200L.1 | 300 × 20 cm | 1 roll(s) |
€259.10 |
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| 200N.1 | 20 × 20 cm | 5 sheet(s) |
€118.25 |
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| 200P.1 | 10 × 10 cm | 10 sheet(s) |
€101.45 |
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In stock
Available
In procurement
No longer available
Delivery date currently unknown
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- Subtotal: 0.00
Downloads / MSDS
General information
Technical Parameters of ROTH-Transfer Membranes
| Membrane | Material | Characteristics | Thickness (μm) | Binding capacity (μg/cm2) | Pore size (μm) | Immobilisation |
| Roti® Fluoro PVDF | Polyvinyliden fluoride | hydrophobic | 125 ±25 | 300 | 0,2 | not necessary |
| Transfer membrane ROTI®PVDF 0.2 | Polyvinyliden fluoride | hydrophobic | 190 ±50 | ≥125 | 0,2 | not necessary |
| Transfer membrane ROTI®PVDF 0.45 | Polyvinyliden fluoride | hydrophobic | 190 ±50 | ≥125 | 0,45 | not necessary |
| Immobilon P | Polyvinyliden fluoride | hydrophobic | 120 ±25 | 150-300 | 0,45 | not necessary |
| Roti® Nylon 0.2 | Nylon 6,6 | neutral/amphoteric | 150 ±20 | 500 | 0,2/0,45 | UV, baking |
| Roti® Nylon plus | Nylon 6,6 | hydrophilic, positively charged | 160 ±20 | 500 | 0,45 | not necessary, UV and baking possible |
| Transfer membrane ROTI®NC 0.2 | Nitrocellulose | hydrophobic | 150 ±50 | 100 | 0,2 | UV, baking (max. 80 °C) |
| Transfer membrane ROTI®NC 0.45 | Nitrocellulose | hydrophobic | 150 ±50 | 100 | 0,45 | UV, baking (max. 80 °C) |
| Amersham Protran® | Nitrocellulose | electrostatic, hydrophobic | unknown | 11-72 | 0,2/0,45 | UV, baking (max. 80 °C) |
| Amersham Protran® supported | Nitrocellulose | electrostatic, hydrophobic | unknown | 86-110 | 0,2/0,45 | UV, baking (max. 80 °C) |
| Biomolecules / Membrane Pores | Size (kD) |
| genomic DNA (human) | >11000000 |
| E. coli | approx. 1500000 |
| 0.45 μm | approx. 5000 |
| 0.2 μm | approx. 2000 |
| DNA 3 kb | approx. 1000 |
| RNA 1 kb | approx. 300 |
| Antibodies (IgG) | approx. 150 |
| Proteins in the lab | approx. 10–300 |
| BSA (Albumin) | 69 |
| Biomolecules | Nylon plus | Nylon 0.2 | PVDF 0.2 | PVDF | Fluoro PVDF | NC | NC 0.45 |
| DNA | +++ | + | - | - | - | + | + |
| RNA | + | +++ | - | - | - | + | + |
| Proteins | - | + | +++ | +++ | +++ | + | + |
| Detection System | |||||||
| Colour | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
| Radioactivity | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
| Chemoluminescence | +++ | +++ | +++ | +++ | - | + | + |
| Fluorescence | +++ | +++ | - | - | +++ | - | - |
| +++: recommended | +: suitable | -: not | |||||